Published on Sep 05, 2023
Antibiotics are the organic secretion produced by micro – organisms, which in low concentrations are antagonistic to the growth of other micro – organisms (mostly pathogens). Antibiotics have proved very useful in combating several bacterial diseases in man an animal. Antibiotics are commonly obtained from actinomycetes and some eubacteria. Some of the important antibiotics are streptomycin, Aureomycin, teramycin, Chloromycetin, erythromycin, neomycin etc.
Soil is a natural medium that harbors several types of micro – organism .These micro- organisms can be frown on culture media. The effect of different types of antibiotics can be studied on the growth of micro - organism growing in culture medium. This is an important subject, therefore the study of effect of antibiotics on micro - organism has been taken for the present project.
The aim of this project is to study the effect of antibiotics on micro – organisms.
To study the effect of antibiotics on micro-organisms
Potato ,agar, dextrose , distilled water , four different types of antibiotics (such as penicillin , streptomycin , aureomycin , teramycin ) , syringe , oven sterilized petridish , flasks , beakers , pipettes , garden soil , glass marker pen , etc
1. Potato Dextrose Agar (PDA) Medium.
Take 200 g of peeled potato chips. Boil them with 500 ml of water in a beaker for 15 minutes.
Squeeze the potato pulp thus obtained through a muslin cloth and keep it in a flask.
Take 20 g of agar in a beaker and warm it with 500 ml of water .
Mix both the solution of potato and agar and add 20 g dextrose to it.
Thus one litre of PDA medium is prepared.
Autoclave the medium at 15 pounds pressure for 15 minutes.
i) Take 2 g of soil and dissolve it in 10 ml of water in a beaker. Let the soil particle settle down.
ii) Take 5 oven sterilized petridishes and pour 1 ml of soil suspension in each of the plates. Now pour 1ml of the four antibiotics separately in four petridishes with the help of syringe, and mark them with marker pen. Leave the fifth petridish without antibiotic to serve as control.
iii) Pour PDA in each of the petridishes and mix the suspension by rotating the petridishes. Leave the petridishes undisturbed at a warm place
The effect of different antibiotics on the micro – organisms can be assessed by counting the number and size of the colonies growing in the petridishes.
Pencillin and Terramycin was most effective antibiotic against microorganism in soil.
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